lentiviral vector plko 1 hygro (Addgene inc)
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Addgene inc
lentiviral vector plko 1 hygro
Lentiviral Vector Plko 1 Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+hygro+lentiviral+vector/pLKO%2E1+hygro+(Plasmid+%2324150)/pmc10068764__pnas__2202815120__sapp-130-1-8
Average 94 stars, based on 142 article reviews
Lentiviral Vector Plko 1 Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+hygro+lentiviral+vector/pLKO%2E1+hygro+(Plasmid+%2324150)/pmc10068764__pnas__2202815120__sapp-130-1-8
Average 94 stars, based on 142 article reviews
lentiviral vector plko 1 hygro - by Bioz Stars,
2026-09
94/100 stars
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Control:Article Title: Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation Article Snippet: .. Short hairpins targeting Peg3 (shPeg3) and a short hairpin control (shCtrl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co- transfected with the lentiviral plasmid of interest (pCW57.1- Luciferase or pCW57.1- mDux- CA) and the viral packing vectors (pCMV-∆R8.9 and pCMV- VSV- G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1- Luciferase and pCW57.1- mDux- CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2), and a scramble control sequence (shScbl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co-transfected with the lentiviral plasmid of interest (pCW57.1-Luciferase or pCW57.1-mDux-CA) and the viral packing vectors (pCMV-∆R8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2), and a scramble control sequence (shScbl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co-transfected with the lentiviral plasmid of interest (pCW57.1-Luciferase or pCW57.1-mDux-CA) and the viral packing vectors (pCMV-ΔR8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2) and a scramble control sequence (shScbl) were cloned into the Article Title: MiR-29-mediated elastin down-regulation contributes to inorganic phosphorus-induced osteoblastic differentiation in vascular smooth muscle cells. Article Snippet: .. To generate the lentivirus shRNA constructs against human elastin and control shRNA, the sequences (below) were cloned into the Age I and EcoRI sites of the Article Title: Mesenchymal stem cells generate distinct functional hybrids in vitro via cell fusion or entosis Article Snippet: The PCR product derived from pH2B-eGFP (Addgene #11680)4 containing part of the H2B tagged with the eGFP was digested with EcoRI/ClaI and subcloned into the lentiviral vector HIV-H2B::mRFP. .. Short hairpins targeting Rock1 (shRock1.1 and shRock1.2), Rock2 (shRock2.1 and shRock2.2) and a short hairpin control (shCTR) were cloned into the Article Title: Wnt/β-catenin signaling pathway safeguards epigenetic stability and homeostasis of mouse embryonic stem cells Article Snippet: .. Short hairpins targeting ß-catenin (shß-cat #1, #2, #3), and a short hairpin control (shCtrl) were cloned into the Clone Assay:Article Title: Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation Article Snippet: .. Short hairpins targeting Peg3 (shPeg3) and a short hairpin control (shCtrl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co- transfected with the lentiviral plasmid of interest (pCW57.1- Luciferase or pCW57.1- mDux- CA) and the viral packing vectors (pCMV-∆R8.9 and pCMV- VSV- G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1- Luciferase and pCW57.1- mDux- CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2), and a scramble control sequence (shScbl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co-transfected with the lentiviral plasmid of interest (pCW57.1-Luciferase or pCW57.1-mDux-CA) and the viral packing vectors (pCMV-∆R8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2), and a scramble control sequence (shScbl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co-transfected with the lentiviral plasmid of interest (pCW57.1-Luciferase or pCW57.1-mDux-CA) and the viral packing vectors (pCMV-ΔR8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2) and a scramble control sequence (shScbl) were cloned into the Article Title: MiR-29-mediated elastin down-regulation contributes to inorganic phosphorus-induced osteoblastic differentiation in vascular smooth muscle cells. Article Snippet: .. To generate the lentivirus shRNA constructs against human elastin and control shRNA, the sequences (below) were cloned into the Age I and EcoRI sites of the Article Title: Mesenchymal stem cells generate distinct functional hybrids in vitro via cell fusion or entosis Article Snippet: The PCR product derived from pH2B-eGFP (Addgene #11680)4 containing part of the H2B tagged with the eGFP was digested with EcoRI/ClaI and subcloned into the lentiviral vector HIV-H2B::mRFP. .. Short hairpins targeting Rock1 (shRock1.1 and shRock1.2), Rock2 (shRock2.1 and shRock2.2) and a short hairpin control (shCTR) were cloned into the Article Title: Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression Article Snippet: .. shRNA oligomers were annealed and cloned into Article Title: Wnt/β-catenin signaling pathway safeguards epigenetic stability and homeostasis of mouse embryonic stem cells Article Snippet: .. Short hairpins targeting ß-catenin (shß-cat #1, #2, #3), and a short hairpin control (shCtrl) were cloned into the Plasmid Preparation:Article Title: Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation Article Snippet: .. Short hairpins targeting Peg3 (shPeg3) and a short hairpin control (shCtrl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co- transfected with the lentiviral plasmid of interest (pCW57.1- Luciferase or pCW57.1- mDux- CA) and the viral packing vectors (pCMV-∆R8.9 and pCMV- VSV- G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1- Luciferase and pCW57.1- mDux- CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2), and a scramble control sequence (shScbl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co-transfected with the lentiviral plasmid of interest (pCW57.1-Luciferase or pCW57.1-mDux-CA) and the viral packing vectors (pCMV-∆R8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2), and a scramble control sequence (shScbl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co-transfected with the lentiviral plasmid of interest (pCW57.1-Luciferase or pCW57.1-mDux-CA) and the viral packing vectors (pCMV-ΔR8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2) and a scramble control sequence (shScbl) were cloned into the Article Title: MiR-29-mediated elastin down-regulation contributes to inorganic phosphorus-induced osteoblastic differentiation in vascular smooth muscle cells. Article Snippet: .. To generate the lentivirus shRNA constructs against human elastin and control shRNA, the sequences (below) were cloned into the Age I and EcoRI sites of the Article Title: Mesenchymal stem cells generate distinct functional hybrids in vitro via cell fusion or entosis Article Snippet: The PCR product derived from pH2B-eGFP (Addgene #11680)4 containing part of the H2B tagged with the eGFP was digested with EcoRI/ClaI and subcloned into the lentiviral vector HIV-H2B::mRFP. .. Short hairpins targeting Rock1 (shRock1.1 and shRock1.2), Rock2 (shRock2.1 and shRock2.2) and a short hairpin control (shCTR) were cloned into the Article Title: Wnt/β-catenin signaling pathway safeguards epigenetic stability and homeostasis of mouse embryonic stem cells Article Snippet: .. Short hairpins targeting ß-catenin (shß-cat #1, #2, #3), and a short hairpin control (shCtrl) were cloned into the Sequencing:Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co- transfected with the lentiviral plasmid of interest (pCW57.1- Luciferase or pCW57.1- mDux- CA) and the viral packing vectors (pCMV-∆R8.9 and pCMV- VSV- G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1- Luciferase and pCW57.1- mDux- CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2), and a scramble control sequence (shScbl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co-transfected with the lentiviral plasmid of interest (pCW57.1-Luciferase or pCW57.1-mDux-CA) and the viral packing vectors (pCMV-∆R8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2), and a scramble control sequence (shScbl) were cloned into the Article Title: SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state Article Snippet: HEK293T cells were co-transfected with the lentiviral plasmid of interest (pCW57.1-Luciferase or pCW57.1-mDux-CA) and the viral packing vectors (pCMV-ΔR8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284). .. Short hairpins targeting Smarcad1 (shSmarcad1), Topbp1 (shTopbp1 #1 and shTopbp1 #2) and a scramble control sequence (shScbl) were cloned into the shRNA:Article Title: MiR-29-mediated elastin down-regulation contributes to inorganic phosphorus-induced osteoblastic differentiation in vascular smooth muscle cells. Article Snippet: .. To generate the lentivirus shRNA constructs against human elastin and control shRNA, the sequences (below) were cloned into the Age I and EcoRI sites of the Article Title: Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression Article Snippet: .. shRNA oligomers were annealed and cloned into Construct:Article Title: MiR-29-mediated elastin down-regulation contributes to inorganic phosphorus-induced osteoblastic differentiation in vascular smooth muscle cells. Article Snippet: .. To generate the lentivirus shRNA constructs against human elastin and control shRNA, the sequences (below) were cloned into the Age I and EcoRI sites of the Article Title: Mesenchymal stem cells generate distinct functional hybrids in vitro via cell fusion or entosis Article Snippet: The PCR product derived from pH2B-eGFP (Addgene #11680)4 containing part of the H2B tagged with the eGFP was digested with EcoRI/ClaI and subcloned into the lentiviral vector HIV-H2B::mRFP. .. Short hairpins targeting Rock1 (shRock1.1 and shRock1.2), Rock2 (shRock2.1 and shRock2.2) and a short hairpin control (shCTR) were cloned into the |